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Giardia special stain

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Debra L. Conversely, while adult cats often have different and more chronic causes of diarrhea than kittens, the condition remains a common reason for cats to be presented to veterinarians for care. This review discusses some of the more common causes as well as the best approaches for diagnosis and treatment of diarrhea in kittens and cats.

The role of diet in diagnosis and therapy of diarrhea is considered when appropriate. Diarrhea in kittens may range in severity from mild and self-limiting to a severe, hemorrhagic, giardia special stain condition. Knowledge of the most frequently encountered causes of diarrhea in kittens is essential in formulating appropriate diagnostic giardia special stain treatment plans for these young patients.

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Although adult cats can also have infectious, parasitic, mechanical e. In many adult cats, Giardia species infections are subclinical or transient, but in kittens, infection is classically associated with an acute onset of malodorous, pale, mucoid diarrhea.

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The diagnosis is relatively straightforward when the trophozoites or cysts are identified on fresh fecal smears or flotation. Treatment of giardiasis in cats and kittens has not changed drastically for many years, and includes specific antiprotozoal therapy combined with environmental control.

A note of caution here is advised using metronidazole at this dose for longer than days, as metronidazole toxicity is much more likely to result. However, the lack of efficacy may have been due to the combined infection, so, the true effectiveness of fenbendazole against Giardia species is not known. Finally, experimentally infected cats were effectively giardia special stain with a combination product giardia special stain febantel Drontal Plus, Bayer Animal Health.

In that study, the kittens did not have diarrhea from Giardia infection, but Giardia antigen test results became negative after therapy, suggesting complete removal of the organism.

One note of caution is that this drug is not approved for use in cats, primarily because neurologic signs were observed during initial testing. Because reinfection is a major cause of persistent or recurrent infection in a household, cattery, or shelter, appropriate environment control measures are essential. Such measures include environmental merlangius merlangus paraziták cleaning all floors, cages, litter pans, and surfaces that have been in contact with feces with quaternary ammonium or Clorox-containing disinfectantscoat cleaning bathing or shaving of long-haired catsand isolation of affected animals during the diarrheic phase to prevent infection through grooming and other forms giardia special stain contact.

Vaccination with the commercially available Giardia vaccine GiardiaVax, Fort Dodge Animal Health, Overland Park, KS does not prevent infection or shedding of cysts but may reduce the severity and the duration of shedding.

As a result of studiesperformed by Lappin, et al, evaluating the effectiveness of this vaccine, it is not recommended for routine use in household cats. Because Giardia is zoonotic, appropriate education about handling infected giardia y oxiuros and kittens is also important.

Trichomoniasis Trichomoniasis, caused by Tritrichomonas foetus, has been recently recognized as a pathogen in kittens and adult cats. This is the same protozoal organism that infects cattle, causing early embryonic death, abortion, and pyometra.

However, in cats, the organism infects the large intestinal mucosa and causes chronic large bowel diarrhea characterized by increased mucus, tenesmus, hematochezia, and increased frequency of defecation. Most affected kittens are healthy, alert, and active. The only outward signs of illness are the presence of anal hyperemia or swelling, and painful defecation.

Most infections are diagnosed in young kittens with chronic diarrhea average age 9 monthsbut infection can occur at any age. Cats that are exposed to the organism will probably become infected, and infection is likely to be persistent. In a recent study, all eight cats that were exposed to Tritrichomonas trophozoites became giardia special stain as evidenced by fecal cultures performed throughout the days of the study.

However, infection with T.

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Diagnosis of this infection can be made by one of four approaches, listed according to relative ease of use and expense: 1 direct examination of the feces for trophozoites, 2 fecal cultures for the organism using the InpouchTF kit, Biomed Diagnostics, White City, OR3 polymerase giardia special stain reaction PCR of feces must be submitted to specific labs, Dr.

However, it is still the easiest and potentially fastest way to make a definitive diagnosis. The InPouchTF kit is more sensitive than the fecal examination but takes up to 12 days to grow the organism for diagnosis. The samples can be sent to a lab for culture, or the culture kits can be obtained and used giardia special stain the practice setting. Because other trichomonad species can grow in the pouch, PCR testing of the cultured organisms may be needed.

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PCR testing is the most sensitive and specific method of diagnosis, but is also more expensive and results take longer to obtain because only a few labs can do this test. Therapy of cats with trichomoniasis is difficult because there is no readily available, approved drug. Metronidazole and other antibiotics have been used in both experimentally and naturally infected cats but are completely ineffective in clearing the infection.

Because members of the nitroimidazole family of antimicrobials would be expected to be effective against trichomonads, other drugs from the family have giardia special stain tested. However, this drug is not approved for use in cats and is potentially hepatotoxic and neurotoxic. Thus, it should only be used when a definitive diagnosis has been made and the owners understand the risks associated with therapy.

It should not be used empirically to treat cats with undiagnosed diarrhea because of numerous anecdotal reports of neurotoxicity, especially at higher-than-recommended doses and for longer than 14 days. All other antibiotics and parasiticidals, including tylosin, enrofloxacin, azithromycin, and fenbendazole, have not giardia special stain shown to be effective against T. Clinical remission has been giardia special stain to occur in many infected cats, usually by 2 years of age, even without treatment.

However, the malodorous feces associated with trichomoniasis reduces the likelihood that infected cats will be acceptable indoor pets. At this time there is no evidence that this organism is zoonotic, but it is certainly infectious to other cats, and appropriate infection prevention measures should be instituted.

Cryptosporidiosis Cryptosporidium parvum is a coccidian parasite that infects the microvilli of the intestinal epithelium of kittens and immunosuppressed cats.

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The disease caused by this infection can range from an asymptomatic carrier state; to mild, transient illness; to prolonged, life-threatening malabsorption syndromes. In some adult cats, the organism can cause intestinal infiltrates similar to those observed with classic IBD, and because the organisms are very small, unless special stains are used, may be not diagnosed.

Because, like many coccidians, the organisms are shed intermittently further complicates the diagnosis because infected cats may have a negative IFA. Because cryptosporidiosis is zoonotic, appropriate precautions, such as wearing gloves and cleaning any utensils with disinfectants bleachmust be used when infected feces are strongyloidosis mik ezek a tünetek. Treatment of kittens and catsinfected with cryptosporidiosis is very difficult, giardia special stain the drugs purported to be effective against this organism are either toxic or ineffective in cats e.

Tylosin, metronidazole, and other commonly used antimicrobials are ineffective in eradicating the organism. They may improve diarrhea if giardia special stain bacterial component is active, but are unhelpful in most cases. Other Infectious Causes of Diarrhea in Kittens Viral causes Other causes of diarrhea that should be considered in kittens include several viral diseases: feline panleukopenia virus, hemorrhagic calicivirus, rotavirus, astrovirus, enteric coronaviruses, and feline infectious peritonitis.

Feline panleukopenia is the most clinically important intestinal virus of this group and affects primarily unvaccinated kittens, causing fever, depression, anorexia, vomiting, and diarrhea.

Diagnosis is usually based on the clinical signs and history, but the canine parvovirus antigen test giardia special stain with the feline virus and thus can be used for confirmation.

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In adult cats, hemorrhagic calicivirus, FeLV, FIV and feline infectious peritonitis virus are the most important viral causes of enteritis and diarrhea. Treatment of this and any viral enteritis in kittens is symptomatic and supportive. Because young kittens are prone to dehydration and hypoglycemia, fluid therapy and nutritional support are key aspects. The definitive diagnosis of bacterial infections as the cause of diarrhea in giardia special stain or cats is very difficult, primarily because pathogens can be giardia special stain in similar rates from both diarrhea and nondiarrheic feces.

Furthermore, many anaerobic bacteria cannot be easily cultured from feces or intraluminal ingesta due to their lability in air, thus, accurate identification of many of these species will likely require giardia special stain advanced techniques or molecular identification methods. Fungal causes Other infectious causes of diarrhea include fungal diseases, such as Histoplasma; however, these infections are more likely to occur in adult cats.

Parasitic causes Most of the parasitic causes of diarrhea in kittens are typical e. However, therapeutic deworming should still be done in kittens that have diarrhea, even if the results of fecal testing is negative.

Anatomical and Mechanical Causes The curious, investigative nature giardia special stain kittens makes them prone to ingesting foreign substances that may cause diarrhea or vomiting. In most cases, obstructions or intussusception cause vomiting, but the more distal the problem in the gastrointestinal tract, the more giardia special stain diarrhea may be the primary clinical sign. Mechanical or anatomic causes of diarrhea to be considered in kittens include developmental abnormalities e.

In general, diagnosis of these conditions requires radiographs or other imaging techniques, but ingestion of string can occasionally be diagnosed by finding the string trapped at the klinika és a helmintiasias kezelése of the tongue or by palpation of the resulting intussusception.

Most of these problems require surgery for resolution, and except in the case of developmental abnormalities, often have a good to excellent prognosis. Nutritional Causes of Diarrhea in Kittens The use of diet to assist in diarrhea management is not a new concept.

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Nevertheless, the type of diet used to help manage the problem has become an increasingly complex issue. In many if not most simple cases of diarrhea especially in kittensthe best approach is to feed a highly digestible diet moderate to low fat, moderate protein, moderate carbohydrate that has fewer food colors, preservatives, flavorings, or other substances than may be associated with food intolerance e.

Some of these highly digestible diets may contain additives designed to improve intestinal health such as soluble fibers, omega-3 fatty acids, prebiotics, and increased antioxidants.

Thus, when one diet from this category not accepted by the kitten, is ineffective or seems to make the diarrhea worse, you cannot assume that all diets in this category will be ineffective. If a highly digestible diet has been fed for at least 2 weeks with minimal response, then is it reasonable to giardia special stain another diet or an entirely different dietary strategy e.

Another consideration in kittens is that the diarrhea may be due to carbohydrate intolerance or bacterial changes resulting from dietary changes i. Thus, in kittens with carbohydrate intolerance, feeding a canned food diet, which contains fewer carbohydrates, may resolve the diarrhea. Alternatively, adding probiotics to the diet may a giardiasis a giardia special stain gyógyszer increase the normal microflora, and is also a reasonable therapeutic option.

Inflammatory or Immune-Mediated Causes Inflammatory Bowel Disease Inflammatory bowel disease IBD is a commonly diagnosed chronic intestinal disorder of adult cats, but it is a condition that frequently represents multiple diseases. IBD is characterized by persistent clinical signs vomiting, diarrhea, or weight loss consistent with gastrointestinal disease that occurs concurrently with histologic evidence of mucosal inflammation. These differentials should be investigated giardia special stain and appropriate empirical therapy should be instituted before a diagnosis of idiopathic IBD can be made.

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Food sensitivity can be particularly difficult to distinguish from IBD or other intestinal disorders. Thus, appropriate food trials are very important in both diagnosis and therapy of cats with gastrointestinal disease or suspected IBD. Serum cobalamin levels in cats commonly decrease with severe small bowel disease, and in cats with hypocobalaminemia, the diarrhea will not resolve until replacement therapy is instituted. Cobalamin therapy µg IM weekly giardia special stain 4 weeks, then giardia special stain prn in some cats may be lifelong, while in others, supplementation can be discontinued once the clinical disease resolves.

Radiographs and ultrasonography are important to evaluate for the presence of such infiltrative diseases as Giardia special stain granulomas, histoplasmosis, or lymphosarcoma. Ultimately, intestinal biopsies, either obtained endoscopically or during laparotomy—are essential both for the diagnosis of IBD and for ruling out other specific causes of the clinical signs.

In humans, recent studies indicate a strong association between IBD and a breakdown of normal tolerance mechanisms, host susceptibility, and enteric microflora.

It is quite likely that these same factors are important in feline IBD, and in studies using experimental models of IBD, the resident microflora are essential cofactors in driving the inflammatory response.

Further, modulation of the enteric microenvironment using probiotic therapy and targeted antimicrobial therapy in humans with IBD has been shown to reduce pathogenic bacteria and reduce proinflammatory cytokines, thereby decreasing the inflammation and resulting in better disease control. Unfortunately, accurate, readily accessible methods of assessing bacterial numbers and the species populating the small intestine are not yet available in clinical cases.

In addition, studies in cats with IBD assessing modulation of the enteric flora using probiotics, prebiotics, or other specific therapy for cytokines are only in the early stages of study.

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At this time, therapy of IBD in cats continues to include inflammatory suppression and antibiotic therapy.